tailieunhanh - Báo cáo khoa học: "An immunohistochemical Study on the Pancreatic Endocrine Cells of the C57BL/6 Mouse"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: An immunohistochemical Study on the Pancreatic Endocrine Cells of the C57BL/6 Mouse | J. Vet. Sci. 2002 3 4 327-333 JOURNAL OF Veterinary Science An immunohistochemical Study on the Pancreatic Endocrine Cells of the C57BL 6 Mouse Sae-Kwang Ku Hyeung-Sik Lee 1 and Jae-Hyun Lee2 Pharmacology Toxicology Lab. Central Research Laboratories Dong-Wha Pharm. Ind. Co 1Department of Biology Faculty of Natural Sciences Kyungsan University 2Department of Histology College of Veterinary Medicine Kyungpook National University Received June 19 2002 Accepted November 28 2002 Abstract The regional distribution and relative frequency of the pancreatic endocrine cells in the C57BL 6 mouse were studied by immunohistochemical method using four types of specific m amm alian antisera against insulin glucagon som atostatin and hum an pancreatic polypeptide PP . The pancreas of mouse could be divided into three portions pancreatic islets pancreatic duct and exocrine portions and pancreatic isle ts were further subdivided into three regions central m antle and peripheral regions according to their located types of immunoreactive cells and pancreatic duct portions were also subdivided into two regions epithelial and connective tissue regions . In the pancreatic isle t portions although some cells were also demonstrated in the m antle regions most of insulin-immunoreactive cells were located in the central regions and they were randomly dispersed in the whole pancreatic islets. Glucagon-immunoreactive ce lls were detected in the m antle and peripheral regions. Their relative frequencies in the peripheral regions were somewhat num erous than those of the m antle regions. Som atostatin-immunoreactive ce lls were detected in the m antle and peripheral regions. However no PP-immunoreactive cells were demonstrated in the pancreatic islets of C57BL 6 m ouse. In the pancreatic duct portions rare glucagon-immunoreactive ce lls were situated in the epithelial regions. Cell clusters that consisted of glucagon- or som atostatin-immunoreactive cells were found in som e case of connective