tailieunhanh - Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane

The assembly status of the cytochromebc1 complex has been analyzed in distinct yeast deletion strains in which genes for one or more of thebc1 subunits were deleted. In all the yeast strains tested, a bc1 sub-complex of approximately 500 kDa was found when the mitochondrial membranes were analyzed by blue native electrophoresis. | Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane Vincenzo Zara1 Laura Conte1 and Bernard L. Trumpower2 1 Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali Universita delSalento Lecce Italy 2 Department of Biochemistry Dartmouth Medical School Hanover NH USA Keywords cytochrome bc1 assembly cytochrome bc1 complex cytochrome bc1 core structure yeast deletion mutants yeast mitochondria Correspondence V. Zara Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali University delSalento Via Prov. le Lecce-Monteroni I-73100 Lecce Italy Fax 39 0832 298626 Tel 39 0832 298705 E-mail Received 17 December 2008 revised 16 January 2009 accepted 20 January 2009 doi The assembly status of the cytochrome bc1 complex has been analyzed in distinct yeast deletion strains in which genes for one or more of the bc1 subunits were deleted. In all the yeast strains tested a bc1 sub-complex of approximately 500 kDa was found when the mitochondrial membranes were analyzed by blue native electrophoresis. The subsequent molecular characterization of this sub-complex carried out in the second dimension by SDS PAGE and immunodecoration revealed the presence of the two catalytic subunits cytochrome b and cytochrome c1 associated with the noncatalytic subunits core protein 1 core protein 2 Qcr7p and Qcr8p. Together these bcl subunits build up the core structure of the cytochrome bc1 complex which is then able to sequentially bind the remaining subunits such as Qcr6p Qcr9p the Rieske iron-sulfur protein and Qcr10p. This bc1 core structure may represent a true assembly intermediate during the maturation of the bc1 complex first because of its wide distribution in distinct yeast deletion strains and second for its characteristics of stability which resemble those of the intact homodimeric bc1 complex. By contrast the bc1 core .

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