tailieunhanh - Báo cáo khoa học: Novel genes in cell cycle control and lipid metabolism with dynamically regulated binding sites for sterol regulatory element-binding protein 1 and RNA polymerase II in HepG2 cells detected by chromatin immunoprecipitation with microarray detection

Sterol regulatory element-binding proteins 1 and 2 (SREBP-1 and SREBP-2) are important regulators of genes involved in cholesterol and fatty acid metabolism, but have also been implicated in the regulation of the cell cycle and have been associated with the pathogenesis of type 2 diabetes, atherosclerosis and obesity, among others. | Novel genes in cell cycle control and lipid metabolism with dynamically regulated binding sites for sterol regulatory element-binding protein 1 and RNA polymerase II in HepG2 cells detected by chromatin immunoprecipitation with microarray detection Mehdi Motallebipour1 Stefan Enroth2 Tanel Punga3 Adam Ameur2 Christoph Koch4 Ian Dunham4 Jan Komorowski2 5 Johan Ericsson3 and Claes Wadelius1 1 Department of Genetics and Pathology Rudbeck Laboratory Uppsala University Sweden 2 Linnaeus Centre for Bioinformatics BiomedicalCentre Uppsala University Sweden 3 Ludwig Institute for Cancer Research BiomedicalCentre Uppsala University Sweden 4 Wellcome Trust Sanger Institute Cambridge UK 5 Interdisciplinary Centre for Mathematicaland Computer Modelling Warsaw University Poland Keywords HCFC1 human intragenic binding sterol-regulated transcriptionalregulation Correspondence C. Wadelius Department of Genetics and Pathology Rudbeck Laboratory Uppsala University SE-75185 Uppsala Sweden Fax 46 18 471 4808 Tel 46 18 471 4076 E-mail Present address Friedrich Miescher Institute for Biomedical Research Basel Switzerland ỊQIAGEN Hamburg GmbH Research Development Hamburg Germany ỊEMBL-European Bioinformatics Institute Wellcome Trust Genome Campus Cambridge UK UCD Conway Institute Schoolof Medicine and MedicalScience University College Dublin Belfield Dublin Ireland Sterol regulatory element-binding proteins 1 and 2 SREBP-1 and SREBP-2 are important regulators of genes involved in cholesterol and fatty acid metabolism but have also been implicated in the regulation of the cell cycle and have been associated with the pathogenesis of type 2 diabetes atherosclerosis and obesity among others. In this study we aimed to characterize the binding sites of SREBP-1 and RNA polymerase II through chromatin immunoprecipitation and microarray analysis in 1 of the human genome as defined by the Encyclopaedia of DNA Elements consortium in a hepatocellular carcinoma cell line .