tailieunhanh - Báo cáo toán học: "Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity"

Tuyển tập các báo cáo nghiên cứu khoa học về toán học trên tạp chí toán học quốc tế đề tài:Role of One N-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity. | Role of One A-linked Oligosaccharide Chain on Canine Herpesvirus gD in Its Biological Activity Ken MAEDA Naoaki YOKOYAMA1 Kentaro FUJITA1 Xuenan XUAN2 and Takeshi MIKAMI1 Department of Veterinary Microbiology Faculty of Agriculture Yamaguchi University 1677-1 Yoshida Yamaguchi 753 Department of Veterinary Microbiology Faculty of Agriculture The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113 and 2-Research Center for Protozoan Molecular Immunology Obihiro University of Agriculture and Veterinary Medicine Inada-cho Obihiro Hokkaido 080 Japan Received 4 June 1997 Accepted 7 August 1997 ABSTRACT. The YP11mu strain of a plaque-selected canine herpesvirus CHV encoded a smaller molecular weight MW of gD than those of other strains including YP2 strain Xuan et al. 1990 . When nucleotide sequence of the mutated gD of YP11mu strain gD YP11mu was compared with that of gDs of other CHV strains gD YP11mu lacked 12 nucleotides encoding 4 amino acids NKTI including one predicted potential V-linked glycosylation site and no other change was found in other regions. When the gD YP11mu and gD of YP2 strain gD YP2 expressed in COS-7 and insect Spodoptera frugiperda Sf9 cells were compared each other both gDs reacted with a panel of monoclonal antibodies MAbs against CHV gD by indirect immunofluorescence analysis and the gD YP11mu possessed an MW of approximately 47-51 and 39-44 kDa in COS-7 and Sf9 cells respectively which were smaller than the expressed gD YP2 approximately 51-55 and 41-46 kDa respectively by immunoblot analysis. After treatment with tunicamycin the MW of both gDs in Sf9 cells became approximately 37 kDa. When hemagglutination HA test using canine red blood cells RBC were carried out lysates of Sf9 cells expressing CHV gDs agglutinated canine RBC. Serum from mice inoculated with lysates of Sf9 cells expressing the gDs possessed a high titer of virus-neutralizing VN activities against CHV. These results indicated that the deletion of 4 amino acids possessing .