tailieunhanh - Báo cáo sinh học: "The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury | Huang et al. Journal of Translational Medicine 2011 9 73 http content 9 1 73 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access The combined transduction of copper zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide PEP-1 to protect myocardium from ischemia-reperfusion injury 1 I- 71 1 1 I X f 1 Guang-Qing Huang Jia-Ning Wang Jun-Ming Tang Lei Zhang Fei Zheng Jian-Ye Yang Ling-Yun Guo Xia Kong1 Yong-Zhang Huang1 Yong Liu2 and Shi-You Chen1 4 Abstract Background Our previous studies indicate that either PEP-1-superoxide dismutase 1 SOD1 or PEP-1-catalase CAT fusion proteins protects myocardium from ischemia-reperfusion-induced injury in rats. The aim of this study is to explore whether combined use of PEP-1-SOD1 and PEP-1-CAT enhances their protective effects. Methods SOD1 PEP-1-SOD1 CAT or PEP-1-CAT fusion proteins were prepared and purified by genetic engineering. In vitro and in vivo effects of these proteins on cell apoptosis and the protection of myocardium after ischemia-reperfusion injury were measured. Embryo cardiac myocyte H9c2 cells were used for the in vitro studies. In vitro cellular injury was determined by the expression of lactate dehydrogenase LDH . Cell apoptosis was quantitatively assessed with Annexin V and PI double staining by Flow cytometry. In vivo rat left anterior descending coronary artery LAD was ligated for one hour followed by two hours of reperfusion. Hemodynamics was then measured. Myocardial infarct size was evaluated by TTC staining. Serum levels of myocardial markers creatine kinase-MB CK-MB and cTnT were quantified by ELISA. Bcl-2 and Bax expression in left ventricle myocardium were analyzed by western blot. Results In vitro PEP-1-SOD1 or PEP-1-CAT inhibited LDH release and apoptosis rate of H9c2 cells. Combined transduction of PEP-1-SOD1 and PEP-1-CAT however further reduced the LDH level and apoptosis rate. In vivo combined usage of PEP-1-SOD1 and PEP-1-CAT .