tailieunhanh - Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction"

ATuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: dipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction | Chen et al. Journal of Translational Medicine 2011 9 51 http content 9 1 51 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction Yen-Ta Chen1t Cheuk-Kwan Sun2 10 Yu-Chun Lin3 4t Li-Teh Chang5 Yung-Lung Chen 3 Tzu-Hsien Tsai3 Sheng-Ying Chung3 Sarah Chua3 Ying-Hsien Kao6 Chia-Hung Yen7 Pei-Lin Shao8 Kuan-Cheng Chang9 Steve Leu3 4 and Hon-Kan Yip3 4 Abstract Background Reactive oxygen species are important mediators exerting toxic effects on various organs during ischemia-reperfusion IR injury. We hypothesized that adipose-derived mesenchymal stem cells ADMSCs protect the kidney against oxidative stress and inflammatory stimuli in rat during renal IR injury. Methods Adult male Sprague-Dawley SD rats n 24 were equally randomized into group 1 sham control group 2 IR plus culture medium only and group 3 IR plus immediate intra-renal administration of X 106 autologous ADMSCs followed by intravenous ADMSCs at 6 h and 24 h after IR . The duration of ischemia was 1 h followed by 72 hours of reperfusion before the animals were sacrificed. Results Serum creatinine and blood urea nitrogen levels and the degree of histological abnormalities were markedly lower in group 3 than in group 2 all p . The mRNA expressions of inflammatory oxidative stress and apoptotic biomarkers were lower whereas the anti-inflammatory anti-oxidative and anti-apoptotic biomarkers were higher in group 3 than in group 2 all p . Immunofluorescent staining showed a higher number of CD31 von Willebrand Factor and heme oxygenase HO -1 cells in group 3 than in group 2 all p . Western blot showed notably higher NAD P H quinone oxidoreductase 1 and HO-1 activities two indicators of anti-oxidative capacity in group 3 than those in group 2 all p . Immunohistochemical staining showed higher glutathione .