tailieunhanh - Báo cáo sinh học: "15 kDa Granulysin versus GM-CSF for monocytes differentiation: analogies and differences at the transcriptome level"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: 15 kDa Granulysin versus GM-CSF for monocytes differentiation: analogies and differences at the transcriptome level | Castiello et al. Journal of Translational Medicine 2011 9 41 http content 9 1 41 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access 15 kDa Granulysin versus GM-CSF for monocytes differentiation analogies and differences at the transcriptome level 1 1 2 3 3 2 Luciano Castiello David F Stroncek Michael W Finn Ena Wang Francesco M Marincola Carol Clayberger Alan M Krensky2 and Marianna Sabatino 1 Abstract Background Granulysin is an antimicrobial and proinflammatory protein with several isoforms. While the 9 kDa isoform is a well described cytolytic molecule with pro-inflammatory activity the functions of the 15 kDa isoform is less well understood. Recently it was shown that 15 kDa Granulysin can act as an alarmin that is able to activate monocytes and immature dendritic cells. Granulocyte Macrophage Colony Stimulating Factor GM-CSF is a growth factor widely used in immunotherapy both for in vivo and ex vivo applications especially for its proliferative effects. Methods We analyzed gene expression profiles of monocytes cultured with 15 kDa Granulysin or GM-CSF for 4 12 24 and 48 hours to unravel both similarities and differences between the effects of these stimulators. Results The analysis revealed a common signature induced by both factors at each time point but over time a more specific signature for each factor became evident. At all time points 15 kDa Granulysin induced immune response chemotaxis and cell adhesion genes. In addition only 15 kDa Granulsyin induced the activation of pathways related to fundamental dendritic cell functions such as co-stimulation of T-cell activation and Th1 development. GM-CSF specifically down-regulated genes related to cell cycle arrest and the immune response. More specifically cytokine production lymphocyte mediated immunity and humoral immune response were down-regulated at late time points. Conclusion This study provides important insights on the effects of a novel agent 15 kDa granulysin .