tailieunhanh - Báo cáo sinh học: " Human neuronal cell protein responses to Nipah virus infection"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Human neuronal cell protein responses to Nipah virus infection | Virology Journal BioMed Central Research Open Access Human neuronal cell protein responses to Nipah virus infection Li-Yen Chang1 AR Mohd Ali2 Sharifah Syed Hassan2 and Sazaly AbuBakar 3 Address 1Center for Proteomics Research Department of Forest Biotechnology Forest Research Institute Malaysia 52109 Selangor Malaysia 2Veterinary Research Institute Jalan Sultan Azlan Shah 13800 Ipoh Perak Malaysia and 3Department of Medical Microbiology Faculty of Medicine University Malaya 50603 Kuala Lumpur Malaysia Email Li-Yen Chang - changliyen@ AR Mohd Ali - ali@ Sharifah Syed Hassan - sharifas@ Sazaly AbuBakar - sazaly@ Corresponding author Published 7 June 2007 Virology Journal 2007 4 54 doi 1743-422X-4-54 Received 16 March 2007 Accepted 7 June 2007 This article is available from http content 4 1 54 2007 Chang et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Nipah virus NiV a recently discovered zoonotic virus infects and replicates in several human cell types. Its replication in human neuronal cells however is less efficient in comparison to other fully susceptible cells. In the present study the SK-N-MC human neuronal cell protein response to NiV infection is examined using proteomic approaches. Results Method for separation of the NiV-infected human neuronal cell proteins using twodimensional polyacrylamide gel electrophoresis 2D-PAGE was established. At least 800 protein spots were resolved of which seven were unique six were significantly up-regulated and eight were significantly down-regulated. Six of these altered proteins were identified using mass spectrometry MS and confirmed using MS MS. The heterogenous nuclear .