tailieunhanh - Báo cáo y học: "Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies. | Open Access Method Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies Essi Laajala Tero Aittokallio Riitta Lahesmaa and Laura L Elo Addresses Turku Centre for Biotechnology University of Turku and Ảbo Akademi University Turku FI-20521 Finland. Department of Mathematics University of Turku Turku FI-20014 Finland. Correspondence Laura L Elo. Email laliel@ Published 16 July 2009 Genome Biology 2009 10 R77 doi gb-2009- 10-7-r77 The electronic version of this article is the complete one and can be found online at http 2009 10 7 R77 Received 16 March 2009 Revised 5 June 2009 Accepted 16 July 2009 2009 Laajala et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The recent advent of exon microarrays has made it possible to reveal differences in alternative splicing events on a global scale. We introduce a novel statistical procedure that takes full advantage of the probe-level information on Affymetrix exon arrays when detecting differential splicing between sample groups. In comparison to existing ranking methods the procedure shows superior reproducibility and accuracy in distinguishing true biological findings from background noise in high agreement with experimental validations. Background Alternative splicing is the process in which multiple mRNA isoforms are generated from a single gene by selectively joining together exons of a primary transcript in different patterns see for example 1 for a review . Thus instead of coding a single protein the same genetic locus may produce a variety of different proteins with different properties and distinct functions in the system. Alternative splicing is emerging as a key mechanism for .