tailieunhanh - Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Purification of infectious human herpesvirus 6A virions and association of host cell proteins | Virology Journal BioMed Central Research Open Access Purification of infectious human herpesvirus 6A virions and association of host cell proteins Maria HammarstedtM Jenny Ahlqvistt2 Steven Jacobson3 Henrik Garoff1 and Anna Fogdell-Hahn 2 Address Department of Biosciences and Nutrition at Novum Karolinska Institutet Huddinge Sweden 2Department of Clinical Neuroscience Division of Neurology Karolinska Institutet Stockholm Sweden and 3National Institute of Neurological Disorders and Stroke National Institutes of Health Bethesda MD USA Email Maria Hammarstedt - Jenny Ahlqvist - Steven Jacobson - JacobsonS@ Henrik Garoff - Anna Fogdell-Hahn - Corresponding author tEqual contributors Published 19 October 2007 Received 15 August 2007 Accepted 19 October 2007 Virology Journal 2007 4 101 doi I743-422X-4-I0I This article is available from http content 4 1 101 2007 Hammarstedt et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Viruses that are incorporating host cell proteins might trigger autoimmune diseases. It is therefore of interest to identify possible host proteins associated with viruses especially for enveloped viruses that have been suggested to play a role in autoimmune diseases like human herpesvirus 6A HHV-6A in multiple sclerosis MS . Results We have established a method for rapid and morphology preserving purification of HHV-6A virions which in combination with parallel analyses with background control material released from mock-infected cells facilitates qualitative and quantitative investigations of the protein content of HHV-6A virions. In our iodixanol

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