tailieunhanh - Báo cáo sinh học: " Cowpea viruses: Effect of single and mixed infections on symptomatology and virus concentration

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Cowpea viruses: Effect of single and mixed infections on symptomatology and virus concentration | Virology Journal BioMed Central Research Open Access Cowpea viruses Effect of single and mixed infections on symptomatology and virus concentration Moni ATaiwo 1 Kehinde T Kareem1 Imade Y Nsa1 and Jackies D A Hughes2 Address 1Dept. of Botany and Microbiology University of Lagos Akoka Lagos Nigeria and international Institute of Tropical Agriculture Ibadan Nigeria Email Moni ATaiwo - monitaiwo@ Kehinde T Kareem - rabkareem2@ Imade Y Nsa - imadee@ Jackies D A Hughes - Corresponding author Published 27 September 2007 Received 10 July 2007 Accepted 27 September 2007 Virology Journal 2007 4 95 doi 1743-422X-4-95 This article is available from http content 4 1 95 2007 Taiwo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Natural multiple viral infections of cultivated cowpeas have been reported in Nigeria. In this study three Nigerian commercial cowpea cultivars Olo 11 Oloyin and White and two lines from the IITA IT86D- 719 and TVU 76 were mechanically inoculated with Cowpea aphid-borne mosaic virus CABMV Bean southern mosaic virus SBMV and Cowpea mottle virus CMeV singly as well as in all possible combinations at 10 20 and 30 days after planting DAP . Samples of leaves or stems were collected at 10 20 and 30 days after inoculation DAI and analyzed for relative virus concentration by Enzyme-Linked Immunosrbent Assay. All the cultivars and lines CVS L were susceptible to the viruses but the commercial CVS showed more severe symptoms and had relatively higher viral concentration. In single virus infections CABMV which induced the most severe symptoms had absorbance values at 405 nm of to while SBMV and CMeV which induced .