tailieunhanh - Báo cáo y học: " Genome-wide investigation of in vivo EGR-1 binding sites in monocytic differentiation"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Genome-wide investigation of in vivo EGR-1 binding sites in monocytic differentiation. | Open Access Genome-wide investigation of in vivo EGR-I binding sites in monocytic differentiation Atsutaka Kubosaki Yasuhiro Tomaru Michihira Tagami Erik Arner Hisashi Miura Takahiro Suzuki Masanori Suzuki Harukazu Suzuki and Yoshihide Hayashizaki Addresses RIKEN Omics Science Center RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan. International Graduate School of Arts and Sciences Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku Yokohama 230-0045 Japan. Correspondence Yoshihide Hayashizaki. Email yosihide@ Published 19 April 2009 Genome Biology 2009 10 R41 doi gb-2009- 10-4-r41 The electronic version of this article is the complete one and can be found online at http 2009 10 4 R41 Received 20 February 2009 Revised 6 April 2009 Accepted 19 April 2009 2009 Kubosaki et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Immediate early genes are considered to play important roles in dynamic gene regulatory networks following exposure to appropriate stimuli. One of the immediate early genes early growth response gene I EGR-1 has been implicated in differentiation of human monoblastoma cells along the monocytic commitment following treatment with phorbol ester. EGR-1 has been thought to work as a modifier of monopoiesis but the precise function of EGR-1 in monocytic differentiation has not been fully elucidated. Results We performed the first genome-wide analysis of EGR-1 binding sites by chromatin immunoprecipitation with promoter array ChIP-chip and identified EGR-1 target sites in differentiating THP-1 cells. By combining the results with previously reported FANTOM4 data we found that EGR-1 binding sites highly .