tailieunhanh - Báo cáo y học: "Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays. | Open Access Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays Johan Staaf David Lindgren Johan Vallon-Christersson Anders Isaksson Hanna Goransson Gunnar Juliusson Richard Rosenquist Mattias Hoglund Ẳke Borg and Markus Ringnér Addresses Department of Oncology Clinical Sciences Lund University SE-22185 Lund Sweden. CREATE Health Strategic Centre for Clinical Cancer Research Lund University SE-22184 Lund Sweden. Department of Medical Sciences Cancer Pharmacology and Informatics Uppsala University SE-75185 Uppsala Sweden. Lund Strategic Research Center for Stem Cell Biology and Cell Therapy Lund University SE-22184 Lund Sweden. Department of Genetics and Pathology Uppsala University SE-75185 Uppsala Sweden. Correspondence Markus Ringnér. Email Published 16 September 2008 Genome Biology 2008 9 RI36 doi gb-2008-9-9-rI 36 The electronic version of this article is the complete one and can be found online at http 2008 9 9 RI36 Received 2 July 2008 Revised 2 September 2008 Accepted I6 September 2008 2008 Staaf et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We present a strategy for detection of loss-of-heterozygosity and allelic imbalance in cancer cells from whole genome single nucleotide polymorphism genotyping data. Using a dilution series of a tumor cell line mixed with its paired normal cell line and data generated on Affymetrix and Illumina platforms including paired tumor-normal samples and tumors characterized by fluorescent in situ hybridization we demonstrate a high sensitivity and specificity of the strategy for detecting both minute and gross allelic imbalances in heterogeneous .