tailieunhanh - Báo cáo y học: "Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon"

Volume et al. Bucca 2009 10, Issue 1, Article R5 Research Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon. | Open Access Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor characterization of the HspR regulon Giselda BuccaH Emma LaingH Vassilis Mersinias Nicholas Allenby Douglas Hurd Jolyon HoldstocV Volker Brenner Marcus Harrison and Colin P Smith Addresses Microbial Sciences Division Faculty of Health and Medical Sciences University of Surrey Guildford GU2 7XH UK. Oxford Gene Technology Ltd Begbroke Business Park Sandy Lane Yarnton Oxford OX5 1PF UK. Current address Institute of Immunology Biomedical Sciences Research Centre Alexander Fleming Athens 16672 Greece. H These authors contributed equally to this work. Correspondence Colin P Smith. Email Published 16 January 2009 Genome Biology 2009 10 R5 doi gb-2009-l 0-l-r5 The electronic version of this article is the complete one and can be found online at http 2009 l0 l R5 Received 2 August 2008 Revised 8 December 2008 Accepted l6 January 2009 2009 Bucca et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background DNA microarrays are a key resource for global analysis of genome content gene expression and the distribution of transcription factor binding sites. We describe the development and application of versatile high density ink-jet in situ-synthesized DNA arrays for the G C rich bacterium Streptomyces coelicolor. High G C content DNA probes often perform poorly on arrays yielding either weak hybridization or non-specific signals. Thus more than one million 60-mer oligonucleotide probes were experimentally tested for sensitivity and specificity to enable selection of optimal probe sets for the genome microarrays. The heat-shock HspR regulatory .