tailieunhanh - Báo cáo y học: "Assignment of isochores for all completely sequenced vertebrate genomes using a consensus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Assignment of isochores for all completely sequenced vertebrate genomes using a consensus. | Open Access Method Assignment of isochores for all completely sequenced vertebrate genomes using a consensus Thorsten Schmidt and Dmitrij Frishman Addresses Department of Genome-Oriented Bioinformatics Wissenschaftszentrum Weihenstephan Technische Universitat Munchen D85350 Freising Germany. Institute for Bioinformatics and Systems Biology MIPS Helmholtz Zentrum Munchen - German Research Center for Environmental Health GmbH Ingolstadter Landstrabe D-85764 Neuherberg Germany. Correspondence Dmitrij Frishman. Email Published 30 June 2008 Genome Biology 2008 9 RI04 doi gb-2008-9-6-rl 04 The electronic version of this article is the complete one and can be found online at http 2008 9 6 R104 Received 13 March 2008 Revised 22 May 2008 Accepted 30 June 2008 2008 Schmidt and Frishman licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We show that although the currently available isochore mapping methods agree on the isochore classification of about two-thirds of the human DNA they produce significantly different results with regard to the location of isochore boundaries and isochore length distribution. We present a new consensus isochore assignment method based on majority voting and provide IsoBase a comprehensive on-line database of isochore maps for all completely sequenced vertebrate genomes. Background More than three decades ago gradient density analyses of fragmented DNA identified long compositionally homogenous regions on mammalian chromosomes widely known as isochores 1-3 or long homogeneous genome regions 4 associated with a wide range of important biological properties. Gene density is up to 16 times higher in GC-rich isochores than in GC-poor isochores 5 with GC .

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