tailieunhanh - Báo cáo y học: "Istituto di Genomica Applicata, Parco Scientifico e Tecnologico di Udine"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Istituto di Genomica Applicata, Parco Scientifico e Tecnologico di Udine. | Open Access Method Annotating genomes with massive-scale RNA sequencing France DenoeudH n Jean-Marc AuryH n Corinne Da Silva Benjamin Noel n Odile Rogier a Massimo Delledonne Michele MorganteU Giorgio Valle Patrick Wincker a Claude Scarpelli n Olivier Jaillon and Frangois Artiguenave n Addresses CEA DSV Institut de Génomique Genoscope 2 rue Gaston Crémieux CP5706 91057 Evry France. CNRS UMR 8030 2 rue Gaston Crémieux CP5706 91057 Evry France. Université d Evry 91057 Evry France. Scientific and Technology Department strada le Grazie 15 37134 Verona Italy. UIstituto di Genomica Applicata Parco Scientifico e Tecnologico di Udine Via Linussio 51 33100 Udine Italy. CRIBI Università degli Studi di Padova viale G. Colombo 35121 Padova Italy. n These authors contributed equally to this work. Correspondence Jean-Marc Aury. Email jmaury@ Published 16 December 2008 Genome Biology 2008 9 R175 doi gb-2008-9- 12-r 175 The electronic version of this article is the complete one and can be found online at http 2008 9 12 R175 Received 9 September 2008 Revised 30 October 2008 Accepted l6 December 2008 2008 Denoeud et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Next generation technologies enable massive-scale cDNA sequencing so-called RNA-Seq . Mainly because of the difficulty of aligning short reads on exon-exon junctions no attempts have been made so far to use RNA-Seq for building gene models de novo that is in the absence of a set of known genes and or splicing events. We present Gene Modelling using RNA-Seq an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome. Background Next generation sequencing .