tailieunhanh - Báo cáo y học: " Identification of transcripts with enriched expression in the developing and adult'

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of transcripts with enriched expression in the developing and adult. | Research Open Access Identification of transcripts with enriched expression in the developing and adult pancreas Brad G Hoffman Bogard Zavaglia Joy Witzsche Teresa Ruiz de Algara Mike Beach Pamela A Hoodlessn Steven JM Jones Marco A Marra and Cheryl D Helgason Addresses Department of Cancer Endocrinology BC Cancer Research Center West 10th Ave Vancouver BC V5Z 1L3 Canada. Terry Fox Laboratory BC Cancer Research Center West 10th Ave Vancouver BC V5Z 1L3 Canada. Department of Medical Genetics Faculty of Medicine University of British Columbia University Boulevard Vancouver BC V6T 1Z3 Canada. Micheal Smith Genome Sciences Centre BC Cancer Agency West 7th Ave Vancouver BC V5Z 4S6 Canada. Department of Surgery Faculty of Medicine University of British Columbia West 10th Avenue Vancouver BC V5Z 4E3 Canada. Correspondence Cheryl D Helgason. Email chelgaso@ Published 14 June 2008 Genome Biology 2008 9 R99 doi 186 gb-2008-9-6-r99 The electronic version of this article is the complete one and can be found online at http 2008 9 6 R99 Received 2 April 2008 Revised 13 May 2008 Accepted 14 June 2008 2008 Hoffman et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Despite recent advances the transcriptional hierarchy driving pancreas organogenesis remains largely unknown in part due to the paucity of comprehensive analyses. To address this deficit we generated ten SAGE libraries from the developing murine pancreas spanning Theiler stages 17-26 making use of available Pdxl enhanced green fluorescent protein EGFP and Neurog3 EGFP reporter strains as well as tissue from adult islets and ducts. Results We used a specificity metric to identify 2 536 tags with pancreas-enriched expression .