tailieunhanh - Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET. | Open Access Research Analysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET Divya MathurH Timothy W DanfordH Laurie A Boyer Richard A Young David K Gifford and Rudolf Jaenisch Addresses Department of Biology Massachusetts Institute of Technology Ames Street Cambridge MA 02139 USA. Whitehead Institute for Biomedical Research Cambridge Center Cambridge MA 02142 USA. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology Vassar Street Cambridge MA 02139 USA. n These authors contributed equally to this work. Correspondence David K Gifford. Email dkg@. Rudolf Jaenisch. Email jaenisch@ Published 13 August 2008 Genome Biology 2008 9 RI26 doi gb-2008-9-8-rI 26 The electronic version of this article is the complete one and can be found online at http 2008 9 8 RI26 Received 7 April 2008 Revised 13 June 2008 Accepted I3 August 2008 2008 Mathur et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genome-wide approaches have begun to reveal the transcriptional networks responsible for pluripotency in embryonic stem ES cells. Chromatin Immunoprecipitation ChIP followed either by hybridization to a microarray platform ChIP-chip or by DNA sequencing ChIP-PET has identified binding targets of the ES cell transcription factors OCT4 and NANOG in humans and mice respectively. These studies have provided an outline of the transcriptional framework involved in maintaining pluripotency. Recent evidence with comparing multiple technologies suggests that expanding these datasets using different platforms would be a useful resource for examining the mechanisms underlying pluripotency regulation. Results We