tailieunhanh - Báo cáo y học: "Reduced-representation methylation mapping"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Reduced-representation methylation mapping. | Minireview Reduced-representation methylation mapping Jeffrey A Jeddeloh John M Greally and Oliver J Rando Addresses Roche NimbleGen Inc. Research and Development 500 S. Rosa Rd Madison WI 53719 USA. fCenter for Epigenomics and Department of Genetics Division of Computational Genetics Albert Einstein College of Medicine 1300 Morris Park Avenue New York NY 10461 USA. Department of Biochemistry and Molecular Pharmacology University of Massachusetts Medical School 364 Plantation St Worcester MA 01605 USA. Correspondence Jeffrey A Jeddeloh. Email . Oliver J Rando. Email Published I September 2008 Genome Biology 2008 9 231 doi gb-2008-9-8-231 The electronic version of this article is the complete one and can be found online at http 2008 9 8 231 2008 BioMed Central Ltd Abstract The power of massively parallel sequencing has been harnessed to map cytosine methylation patterns in the mouse genome allowing insights into the relationship of methylation with DNA sequence histone modifications transcriptional activity and dynamic changes in methylation status during differentiation. The past few decades have seen a revolution in the understanding of mechanisms of epigenetic inheritance - the passing on from one generation of cells to another of information that affects phenotype but does not alter the actual DNA sequence. One of the longest known epigenetic modifications is the methylation of cytosines in DNA which is typical of organisms with large genomes whose cells undergo many divisions over the organism s lifetime. In recent years the realization that inappropriate methylation of promoters of tumor suppressor genes may contribute to oncogenesis has sparked renewed interest in DNA methylation. Despite many years of work however how changes in DNA methylation status occur during normal cell differentiation and whether these changes have a role in regulating gene expression still remain unclear. .