tailieunhanh - Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells. | Open Access Research A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells Wei Wang and Allan Bradley- Addresses Department of Cell Biology and Genetics College of Life Sciences Peking University Beijing 100871 PR China. The Wellcome Trust Sanger Institute Hinxton Cambridge CB10 1SA UK. Correspondence Allan Bradley. Email abradley@ Published 3 April 2007 Genome Biology 2007 8 R48 doi 186 gb-2007-8-4-r48 The electronic version of this article is the complete one and can be found online at http 2007 8 4 R48 Received 15 November 2006 Revised 19 February 2007 Accepted 3 April 2007 2007 Wang and Bradley licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Host factors required for retroviral infection are potential targets for the modulation of diseases caused by retroviruses. During the retroviral life cycle numerous cellular factors interact with the virus and play an essential role in infection. Cultured embryonic stem ES cells are susceptible to retroviral infection therefore providing access to all of the genes required for this process to take place. In order to identify the host factors involved in retroviral infection we designed and implemented a scheme for identifying ES cells that are resistant to retroviral infection and subsequent cloning of the mutated gene. Results A library of mutant ES cells was established by genome-wide insertional mutagenesis in B m-deficient ES cells and a screen was performed by superinfection of the library at high multiplicity with a recombinant retrovirus carrying a positive and negative selection cassette. Stringent negative selection was .