tailieunhanh - Báo cáo y học: "AGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts. | Open Access Method CAGE-TSSchip promoter-based expression profiling using the 5 -leading label of capped transcripts Shintaro Katayama Mutsumi Kanamori-Katayama Kazumi Yamaguchi Piero Carninci and Yoshihide Hayashizaki Addresses Laboratory for Genome Exploration Research Group Genomic Sciences Center RIKEN Yokohama Institute Suehiro-cho Tsurumi-ku Yokohama 230-0045 Japan. Tioinformatics Solutions Division Nittetsu Hitachi Systems Engineering Inc. Akashi-cho Chuo-ku Tokyo 104-6591 Japan. Genome Science Laboratory Discovery and Research Institute RIKEN Wako Main Campus Hirosawa Wako 3510198 Japan. n These authors contributed equally to this work. Correspondence Yoshihide Hayashizaki. Email rgscerg@ Published 26 March 2007 Genome Biology 2007 8 R42 doi 186 gb-2007-8-3-r42 The electronic version of this article is the complete one and can be found online at http 2007 8 3 R42 Received 4 October 2006 Revised 5 January 2007 Accepted 26 March 2007 2007 Katayama et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Cap analysis gene expression CAGE technology has revealed numerous transcription start sites TSSs in mammals and has suggested complex promoter-based patterns of regulation. We developed the CAGE-TSSchip to detect promoter-based transcriptional activity. The CAGE-TSSchip is a customized oligonucleotide array that targets known TSSs identified by CAGE. A new labeling method labeling capped transcripts from the 5 -end had to be developed. The CAGE-TSSchip is accurate and sensitive and represents the activity of each TSS. Background Many genome sequencing projects of model species are finished and a large number of full-length cDNAs have been isolated. Trends in large-scale life .