tailieunhanh - Báo cáo y học: "Fast genomic μChIP-chip from 1,000 cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Fast genomic μChIP-chip from 1,000 cells. | Method Fast genomic pChiP-chip from 1 000 cells John Arne Dahl Andrew H Reiner and Philippe Collas Open Access Address Institute of Basic Medical Sciences Department of Biochemistry Faculty of Medicine University of Oslo 0317 Oslo Norway. Correspondence Philippe Collas. Email Published 10 February 2009 Genome Biology 2009 10 R13 doi gb-2009- l0-2-rl3 The electronic version of this article is the complete one and can be found online at http 2009 l0 2 Rl3 Received 14 November 2008 Revised 16 January 2009 Accepted l0 February 2009 2009 Dahl et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation ChIP assays. These assays are however time-consuming and require large numbers of cells hindering their application to the analysis of many interesting cell types. We report here a fast microChIP pChIP assay for l 000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. pChIP-chip reliably reproduces data obtained by large-scale assays H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed. Background Chromatin immunoprecipitation ChIP has been widely used to analyze the location of post-translationally modified histones or transcription factors in a genome in vivo 1-4 . ChIP analysis of DNA-protein interactions has led to significant advances in the understanding of gene regulation and of how epigenetic phenomena are regulated to affect gene expression DNA repair and replication 5 6 . In a typical ChIP assay large numbers of cells are used DNA and proteins are .