tailieunhanh - Báo cáo sinh học: "Novel non-viral method for transfection of primary leukemia cells and cell lines"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Novel non-viral method for transfection of primary leukemia cells and cell lines | Genetic Vaccines and Therapy BioMed Central Research Open Access Novel non-viral method for transfection of primary leukemia cells and cell lines Frank Schakowski1 Peter Buttgereit1 Martin Mazur1 Angela Marten2 Bjorn Schottker3 Marcus Gorschluter1 and Ingo GH Schmidt-Wolf 1 Address 1Medizinische Klinik und Poliklinik I Rheinische Friedrich-Wilhelms-Universitat Bonn Germany 2Present address Chirurgische Klinik Universitat Heidelberg Germany and 3Present address Med. Klinik Universitat Wurzburg Germany Email Frank Schakowski - F_schakowski@ Peter Buttgereit - pbuttgereit@ Martin Mazur - Angela Marten - Bjorn Schottker - Schoettker_B@ Marcus Gorschluter- Gorschlueter@ Ingo GH Schmidt-Wolf - picasso@ Corresponding author Published 12 January 2004 Received 26 September 2003 Accepted 12 January 2004 Genetic Vaccines and Therapy 2004 2 1 This article is available from http content 2 l l 2004 Schakowski et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Tumor cells such as leukemia and lymphoma cells are possible targets for gene therapy. However previously leukemia and lymphoma cells have been demonstrated to be resistant to most of non-viral gene transfer methods. Methods The aim of this study was to analyze various methods for transfection of primary leukemia cells and leukemia cell lines and to improve the efficiency of gene delivery. Here we evaluated a novel electroporation based technique called nucleofection. This novel technique uses a combination of special electrical parameters and specific solutions to deliver the DNA directly to the cell nucleus under mild conditions. Results Using this technique for gene transfer