tailieunhanh - Báo cáo sinh học: "DNA vaccines: improving expression of antigens"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: DNA vaccines: improving expression of antigens | Genetic Vaccines and Therapy BioMed Central Review DNA vaccines improving expression of antigens Helen S Garmory 1 Katherine A Brown2 and Richard W Titball1 3 Open Access Address 1Dstl Chemical and Biological Sciences Porton Down Salisbury SP4 0JQ UK 2Department of Biological Sciences Centre for Molecular Microbiology and Infection Imperial College of London London SW7 2AZ UK and 3Department of Infectious and Tropical Diseases London School of Hygiene and Tropical Medicine London WC1E 7HT UK Email Helen S Garmory - hsgarmory@ Katherine A Brown - Richard W Titball - RTITBALL@ Corresponding author Published 16 September 2003 Received 04 August 2003 Genetic Vaccines and Therapy 2003 1 2 Accepted 16 September 2003 This article is available from http content 1 1 2 2003 Garmory et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract DNA vaccination is a relatively recent development in vaccine methodology. It is now possible to undertake a rational step-by-step approach to DNA vaccine design. Strategies may include the incorporation of immunostimulatory sequences in the backbone of the plasmid co-expression of stimulatory molecules utilisation of localisation secretory signals and utilisation of the appropriate delivery system for example. However another important consideration is the utilisation of methods designed to optimise transgene expression. In this review we discuss the importance of regulatory elements kozak sequences and codon optimisation in transgene expression. Review In 1990 the direct gene transfer of plasmid DNA into mouse muscle in vivo without the need for a special delivery system was demonstrated 1 . Furthermore intramuscular inoculation with plasmid DNA encoding reporter genes induced protein .