tailieunhanh - Báo cáo sinh học: "Liposomal delivery of p-ialB and p-omp25 DNA vaccines improves immunogenicity but fails to provide full protection against B. melitensis challenge"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Liposomal delivery of p-ialB and p-omp25 DNA vaccines improves immunogenicity but fails to provide full protection against B. melitensis challenge | Commander et al. Genetic Vaccines and Therapy 2010 8 5 http content 8 1 5 GENETIC VACCINES AND THERAPY RESEARCH Open Access Liposomal delivery of p-ialB and p-omp25 DNA vaccines improves immunogenicity but fails to provide full protection against B. melitensis challenge 1 2 3 1 1 Nicola J Commander James M Brewer Brendan W Wren Stephen A Spencer Alastair P MacMillan Judith A Stack1 Abstract Background We have previously demonstrated protective efficacy against B. melitensis using formulations of naked DNA vaccines encoding genes ialB and omp25. The present study was undertaken to further understand the immune response generated by the protective vaccination regimens and to evaluate cationic liposome adsorption as a delivery method to improve vaccine utility. Methods The protective efficacy and immunogenicity of vaccines delivered as four doses of naked DNA a single dose of naked DNA or a single dose of DNA surface adsorbed to cationic liposomes were compared using the BALB c murine infection model of B. melitensis. Antigen-specific T cells and antibody responses were compared between the various formulations. Results The four dose vaccination strategy was confirmed to be protective against B. melitensis challenge. The immune response elicited by the various vaccines was found to be dependent upon both the antigen and the delivery strategy with the IalB antigen favouring CD4 T cell priming and Omp25 antigen favouring CD8 . Delivery of the p-ialB construct as a lipoplex improved antibody generation in comparison to the equivalent quantity of naked DNA. Delivery of p-omp25 as a lipoplex altered the profile of responsive T cells from CD8 to CD4 dominated. Under these conditions neither candidate delivered by single dose naked DNA or lipoplex vaccination methods was able to produce a robust protective effect. Conclusions Delivery of the p-omp25 and p-ialB DNA vaccine candidates as a lipoplex was able to enhance antibody production and effect CD4 T .

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