tailieunhanh - Báo cáo sinh học: "Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant | Jalilian et al. Genetic Vaccines and Therapy 2010 8 4 http content 8 1 4 GENETIC VACCINES AND THERAPY RESEARCH Open Access Development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant Babak Jalilian 1 Abdul Rahman Omar 1 2 Mohd Hair Bejo2 Noorjahan Banu Alitheen3 Mehdi Rasoli1 and Sohkichi Matsumoto4 Abstract Background Studies have shown that DNA vaccines can induce protective immunity which demonstrated the high potential of DNA vaccines as an alternative to inactivated vaccines. Vaccines are frequently formulated with adjuvants to improve their release delivery and presentation to the host immune system. Methods The H5 gene of H5N1 virus A Ck Malaysia 5858 04 was cloned separately into vector. The immunogenicity of the cloned H5 DNA vaccine was tested on SPF chickens using two different approaches. First approach was using H5 DNA vaccine H5 and the second was using H5 DNA vaccine in addition to the MDP1 vaccine. Ten days old chickens inoculated three times with two weeks intervals. The spleen and muscle samples from chickens immunized with H5 H5 and H5 MDP1 H5 MDP1 vaccines were collected after sacrificing the chickens and successfully expressed H5 and MDP1 RNA transcripts. The sera of immunized chickens were collected prior to first immunization and every week after immunization and analyzed using enzyme-linked immunosorbent assay ELISA and hemagglutination inhibition HI test. Results Results of competitive ELISA showed successful antibody responses two weeks post immunization. The HI test showed an increased in antibody titers during the course of experiment in group immunized with H5 and H5 MDP1 vaccines. The result showed that the constructed DNA vaccines were able to produce detectable antibody titer in which the group immunized with H5 MDP1 vaccine produced higher antibody comparing to H5 vaccine alone. Conclusions This study shows for

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