tailieunhanh - Báo cáo sinh học: "Comparison of electrically mediated and liposome-complexed plasmid DNA delivery to the skin"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Comparison of electrically mediated and liposome-complexed plasmid DNA delivery to the skin | Genetic Vaccines and Therapy BioMed Central Research Comparison of electrically mediated and liposome-complexed plasmid DNA delivery to the skin Loree C Heller1 2 Mark J Jaroszeski1 3 Domenico Coppola4 and Richard Heller 1 2 5 Open Access Address 1Center for Molecular Delivery University of South Florida Tampa FL USA 2Frank Reidy Research Center for Bioelectrics Old Dominion University Norfolk VA USA 3Department of Chemical Engineering University of South Florida Tampa FL USA 4Department of Oncologic Sciences H. Lee Moffitt Cancer Center and Research Institute Tampa FL USA and 5Department of Molecular Medicine University of South Florida Tampa FL USA Email Loree C Heller - lheller@ Mark J Jaroszeski - mjarosze@ Domenico Coppola - Richard Heller - rheller@ Corresponding author Published 4 December 2008 Received 14 July 2008 T J_ m I in 1 .-rn nrrz Accepted 4 December 2008 Genetic Vaccines and Therapy 2008 6 16 doi 1479-0556-6-16 This article is available from http content 6 1 16 2008 Heller et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo including the skin. We have previously demonstrated efficient delivery of plasmid DNA to the skin utilizing a custom-built four-plate electrode. The experiments described here further evaluate cutaneous plasmid delivery using in vivo electroporation. Plasmid expression levels are compared to those after liposome mediated delivery. Methods Enhanced electrically-mediated delivery and less extensively liposome complexed delivery of a plasmid encoding the reporter luciferase was tested in .