tailieunhanh - Báo cáo sinh học: "Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles | Genetic Vaccines and Therapy BioMed Central Research Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles Petr Orság1 Veronika Kvardová1 Milan Raska2 Andrew D Miller3 Miroslav Ledvina4 and Jaroslav Turánek 1 Address Veterinary Research Institute Department of Immunology Brno Czech Republic 2Palacky University Faculty of Medicine and Dentistry Department of Immunology Olomouc Czech Republic 3Imperial College Genetic Therapies Centre Department of Chemistry Imperial College London London SW7 2AZ UK and 4The Institute of Organic Chemistry and Biochemistry Prague Czech Republic Email Petr Orság - Veronika Kvardová - Milan Raska - raskamil@ Andrew D Miller - Miroslav Ledvina - ledvina@ Jaroslav Turánek - turanek@ Corresponding author Open Access Published 2 September 2008 Received 15 May 2008 _ .A 11 J . IA I mzn .Im Ai-rz z II Accepted 2 September 2008 Genetic Vaccines and Therapy 2008 6 11 doi 1479-0556-6- II This article is available from http content 6 1 11 2008 Orság et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Application of plasmid DNA for immunization of food-producing animals established new standards of food safety. The addition of foreign products . pDNA into the food chain should be carefully examined to ensure that neither livestock animals nor consumers develop unpredicted or undesirable side-effects. Methods A quantitative real-time PCR QRTPCR methodology was developed to study the biodistribution and persistence of plasmid DNA vaccine .

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