tailieunhanh - Báo cáo sinh học: "Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Differential intracellular distribution of DNA complexed with polyethylenimine (PEI) and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells | Genetic Vaccines and Therapy BioMed Central Research Differential intracellular distribution of DNA complexed with polyethylenimine PEI and PEI-polyarginine PTD influences exogenous gene expression within live COS-7 cells Stephen R Doyle and Chee Kai Chan Open Access Address Department of Genetics and Human Variation La Trobe University Melbourne Victoria 3086 Australia Email Stephen R Doyle - Chee Kai Chan - Corresponding author Published 26 November 2007 Received 22 August 2007 J nrr r II Accepted 26 November 2007 Genetic Vaccines and Therapy 2007 5 11 doi 1479-0556-5-11 This article is available from http content 5 1 11 2007 Doyle and Chan licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Polyethylenimine PEI is one of the most efficient and versatile non-viral vectors available for gene delivery. Despite many advantages over viral vectors PEI is still limited by lower transfection efficiency compared to its viral counterparts. Considerable investigation is devoted to the modification of PEI to incorporate virus-like properties to improve its efficacy including the incorporation of the protein transduction domain PTD polyarginine Arg itself demonstrated to facilitate membrane translocation of molecular cargo. There is however limited understanding of the underlying mechanisms of gene delivery facilitated by both PEI and PEI-bioconjugates such as PEI-polyarginine PEI-Arg within live cells which once elucidated will provide valuable insights into the development of more efficient non-viral gene delivery vectors. Methods PEI and PEI-Arg were investigated for their ability to facilitate DNA internalization and gene expression .