tailieunhanh - Báo cáo sinh học: "Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine | Genetic Vaccines and Therapy BioMed Central Research Open Access Sustained protective rabies neutralizing antibody titers after administration of cationic lipid-formulated pDNA vaccine Michal Margalith and Adrian Vilalta Address Vical Incorporated 10390 Pacific Center Ct San Diego CA 92121 USA Email Michal Margalith - mmargalith@ Adrián Vilalta - avilalta@ Corresponding author Published 15 February 2006 Received 27 January 2006 Accepted 15 February 2006 Genetic Vaccines and Therapy2006 4 2 doi l479-0556-4-2 This article is available from http content 4 1 2 2006Margalith and Vilalta licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein CVS strain was evaluated in vivo for ability to elicit neutralizing titers. pDNA DMRIE-DOPE formulated at two DNA cationic lipid molar ratios was compared in mice to a Vaxfectin -pDNA formulation. Mouse data indicate that Vaxfectin is more effective than DMRIE-DOPE in eliciting neutralizing titers. In addition the ratio of pDNA to DMRIE-DOPE can also affect neutralizing titers. Our data show that sustained neutralizing titers 120 days can be obtained after a single administration of DMRIE-DOPE-formulated pDNA in rabbits. Introduction Each year rabies kills more than 50 000 people and millions of animals worldwide 1 . Although vaccination of dogs elimination of strays and control of wild animals have been effective in preventing most cases of human rabies in .

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