tailieunhanh - Báo cáo sinh học: "Evaluation of the VP22 protein for enhancement of a DNA vaccine against anthrax"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Evaluation of the VP22 protein for enhancement of a DNA vaccine against anthrax | Genetic Vaccines and Therapy BioMed Central Research Open Access Evaluation of the VP22 protein for enhancement of a DNA vaccine against anthrax Stuart D Perkins 1 Helen C Flick-Smith1 Helen S Garmory1 Angela E Essex-Lopresti1 Freda K Stevenson2 and Robert J Phillpotts1 Address Biomedical Sciences Department Defence Science and Technology Laboratory Porton Down Salisbury Wiltshire SP4 OJQ UK and 2Tenovus Laboratory University of Southampton Hospital NHS Trust Southampton SO 16 6YD UK Email Stuart D Perkins - sdperkins@ Helen C Flick-Smith - hcfsmith@ Helen S Garmory - hsgarmory@ Angela E Essex-Lopresti - aeelopresti@ Freda K Stevenson - fs@ Robert J Phillpotts - bjphillpotts@ Corresponding author Published 20 April 2005 Received 13 January 2005 Genetic Vaccines and Therapy 2005 3 3 doi 1479-0556-3-3 Accepted 20 April 2005 This article is available from http content 3 1 3 2005 Perkins et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Previously antigens expressed from DNA vaccines have been fused to the VP22 protein from Herpes Simplex Virus type I in order to improve efficacy. However the immune enhancing mechanism of VP22 is poorly understood and initial suggestions that VP22 can mediate intercellular spread have been questioned. Despite this fusion of VP22 to antigens expressed from DNA vaccines has improved immune responses particularly to non-secreted antigens. Methods In this study we fused the gene for the VP22 protein to the gene for Protective Antigen PA from Bacillus anthracis the causative agent of anthrax. Protective immunity against infection with B. anthracis is almost entirely based on a .