tailieunhanh - Báo cáo sinh học: "Comparison of bovine leukemia virus (BLV) and CMV promoter-driven reporter gene expression in BLV-infected and non-infected cells"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Comparison of bovine leukemia virus (BLV) and CMV promoter-driven reporter gene expression in BLV-infected and non-infected cells | Genetic Vaccines and Therapy BioMed Central Research Open Access Comparison of bovine leukemia virus BLV and CMV promoter-driven reporter gene expression in BLV-infected and non-infected cells Jerome S Harms 1 Kurt A Eakle2 Lillian S Kuo1 Robert D Bremel3 and Gary A Splitter1 Address department of Animal Health and Biomedical Sciences University of Wisconsin-Madison Madison WI 53706-1581 USA 2GALA Biotech 8137 Forsythia Street Middleton WI 53562 USA and 3 IoGenetics LLC 3591 Anderson St. Suite 218 Madison WI 53704 USA Email Jerome S Harms - harms@ Kurt A Eakle - Lillian S Kuo - Kuo@ Robert D Bremel - bremel@ Gary A Splitter - splitter@ Corresponding author Published 24 August 2004 Received 13 May 2004 Accepted 24 August 2004 Genetic Vaccines and Therapy 2004 2 1 I doi 1479-0556-2- II This article is available from http content 2 1 11 2004 Harms et al licensee BioMed Central Ltd. This is an open-access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Viral promoters are used in mammalian expression vectors because they generally have strong activity in a wide variety of cells of differing tissues and species. Methods The utility of the BLV LTR promoter BLVp for use in mammalian expression vectors was investigated through direct comparison to the CMV promoter CMVp . Promoter activity was measured using luciferase assays of cell lines from different tissues and species stably transduced with BLVp or CMVp driven luciferase vectors including D17 FLK and primary bovine B cells. Cells were also modified through the addition of BLV Tax expression vectors and or BLV infection as well as treatment with trichostatin A TSA . Results .