tailieunhanh - báo cáo hóa học:" Vasoprotective effects of human CD34+ cells: towards clinical applications"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : Vasoprotective effects of human CD34+ cells: towards clinical applications | Journal of Translational Medicine BioMed Central Research Vasoprotective effects of human CD34 cells towards clinical applications Thomas J Kiernan1 Barry A Boilson1 Tyra A Witt1 Allan B Dietz2 Amir Lerman1 and Robert D Simari 1 Open Access Address 1Division of Cardiovascular Diseases Mayo Clinic 200 First Street SW Rochester MN 55905 USA and 2Division of Transfusion Medicine Mayo Clinic 200 First Street SW Rochester MN 55905 USA Email Thomas J Kiernan - Barry A Boilson - Tyra A Witt - Allan B Dietz - Amir Lerman - Robert D Simari - Corresponding author Published 29 July 2009 Received 1 May 2009 Journal of Translational Medicine 2009 7 66 doi 1479-5876-7-66 Accepted 29 July 2009 This article is available from http content 7 1 66 2009 Kiernan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The development of cell-based therapeutics for humans requires preclinical testing in animal models. The use of autologous animal products fails to address the efficacy of similar products derived from humans. We used a novel immunodeficient rat carotid injury model in order to determine whether human cells could improve vascular remodelling following acute injury. Methods Human CD34 cells were separated from peripheral buffy coats using automatic magnetic cell separation. Carotid arterial injury was performed in male Sprague-Dawley nude rats using a 2F Fogarty balloon catheter. Freshly harvested CD34 cells or saline alone was administered locally for 20 minutes by endoluminal instillation. Structural and functional analysis of the arteries was .