tailieunhanh - Báo cáo hóa học: "DNA polymeraseh protein expression predicts treatment response and survival of metastatic gastric adenocarcinoma patients treated with oxaliplatin-based chemotherapy"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: DNA polymeraseh protein expression predicts treatment response and survival of metastatic gastric adenocarcinoma patients treated with oxaliplatin-based chemotherapy | Teng et al. Journal of Translational Medicine 2010 8 126 http content 8 1 126 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access DNA polymeraseh protein expression predicts treatment response and survival of metastatic gastric adenocarcinoma patients treated with oxaliplatin-based chemotherapy 7 I 7 1 13 Kai-yuan Teng Miao-zhen Qiu Zhuang-hua Li Hui-yan Luo Zhao-lei Zeng Rong-zhen Luo Hui-zhong Zhang1 3 Zhi-qiang Wang1 2 Yu-hong Li1 2 Rui-hua Xu1 2 Abstract Background DNA polymerase h pol h is capable of bypassing DNA adducts produced by cisplatin or oxaliplatin and is associated with cellular tolerance to platinum. Previous studies showed that defective pol h resulted in enhanced cisplatin or oxaliplatin sensitivity in some cell lines. The purpose of the present study was to investigate the role of pol h protein expression in metastatic gastric adenocarcinoma. Methods Four gastric adenocarcinoma cell lines were chosen to explore the relationship between pol h protein expression and oxaliplatin sensitivity by western blotting and MTT assay. Eighty metastatic gastric adenocarcinoma patients treated with FOLFOX or XELOX regimen as first-line chemotherapy were analyzed corresponding pretreatment formalin-fixed paraffin-embedded tumor tissues were used to detect pol h protein expression by immunohistochemistry. Relationship between pol h protein expression and clinical features and outcome of these patients was analyzed. Results A positive linear relationship between pol h protein expression and 48 h IC50 values of oxaliplatin in four gastric cancer cell lines was observed. Positivity of pol h protein expression was strongly associated with poor treatment response as well as shorter survival at both univariate 8 versus 14 months P and multivariate hazard ratio 95 confidence interval P analysis in eighty metastatic gastric adenocarcinoma patients. Conclusions Our study indicates that polh