tailieunhanh - Báo cáo hóa học: " Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies | Bonanno et al. Journal of Translational Medicine 2010 8 114 http content 8 1 114 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Effects of pegylated G-CSF on immune cell number and function in patients with gynecological malignancies 1 1 1 1 1 2 Giuseppina Bonanno Annabella Procoli Andrea Mariotti Maria Corallo Alessandro Perillo Silvio Danese Raimondo De Cristofaro3 Giovanni Scambia1 Sergio Rutella4 5 Abstract Background Pegylated granulocyte colony-stimulating factor G-CSF pegfilgrastim is a longer-acting form of G-CSF whose effects on dendritic cell DC and regulatory T cell Treg mobilization and on the in vivo and ex vivo release of immune modulating cytokines remain unexplored. Methods Twelve patients with gynecological cancers received carboplatin paclitaxel chemotherapy and singledose pegfilgrastim as prophylaxis of febrile neutropenia. Peripheral blood was collected prior to pegfilgrastim administration day 0 and on days 7 11 and 21 to quantify immunoregulatory cytokines and to assess type 1 DC DC1 type 2 DC DC2 and Treg cell mobilization. In vitro-differentiated monocyte-derived DC were used to investigate endocytic activity expression of DC maturation antigens and ability to activate allogeneic T-cell proliferation. Results Pegfilgrastim increased the frequency of circulating DC1 and DC2 precursors. In contrast CD4 FoxP3 bona fide Treg cells were unchanged compared with baseline. Serum levels of hepatocyte growth factor and interleukin IL -12p40 but not transforming growth factor-p1 or immune suppressive kynurenines significantly increased after pegfilgrastim administration. Interestingly pegfilgrastim fostered in vitro monocytic secretion of IL-12p40 and IL-12p70 when compared with unconjugated G-CSF. Finally DC populations differentiated in vitro after clinical provision of pegfilgrastim were phenotypically mature possessed low endocytic activity and incited a robust T-cell proliferative response. Conclusions .