tailieunhanh - Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma | Qiu et al. Journal of Translational Medicine 2010 8 107 http content 8 1 107 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma -7Ị- 71 Miao-zhen Qiu Zhuang-hua Li Zhi-wei Zhou Yu-hong Li Zhi-qiang Wang Feng-hua Wang Peng Huang 4 Fahad Aziz5 Dao-yuan Wang6 Rui-hua Xu1 2 Abstract Background The existence of circulating tumor cells CTCs in peripheral blood as an indicator of tumor recurrence has not been clearly established particularly for gastric cancer patients. We conducted a retrospective analysis of the relationship between CTCs in peripheral blood at initial diagnosis and clinicopathologic findings in patients with gastric carcinoma. Methods Blood samples were obtained from 123 gastric carcinoma patients at initial diagnosis. mRNA was extracted and amplified for carcinoembryonic antigen CEA mRNA detection using real-time RT-PCR. Periodic 3-month follow-up examinations included serum CEA measurements and imaging. Results The minimum threshold for corrected CEA mRNA score CEA mRNA GAPDH mRNA X 106 was set at 100. Forty-five of 123 patients were positive for CEA mRNA expression. CEA mRNA expression significantly correlated with T stage and postoperative recurrence status P . Recurrent disease was found in 44 of 123 cases and 25 of these were positive for CEA mRNA. Of these patients CEA mRNA was more sensitive than serum CEA in indicating recurrence. Three-year disease-free survival of patients positive for CEA mRNA was significantly poorer than of patients negative for CEA mRNA P . Only histological grade and CEA mRNA positivity were independent factors for disease-free survival using multivariate analysis. Conclusions CEA mRNA copy number in peripheral blood at initial diagnosis was .