tailieunhanh - Báo cáo hóa học: "Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Exhaustive expansion: A novel technique for analyzing complex data generated by higherorder polychromatic flow cytometry experiments | Siebert et al. Journal of Translational Medicine 2010 8 106 http content 8 1 106 JOURNAL OF TRANSLATIONAL MEDICINE METHODOLOGY Open Access Exhaustive expansion A novel technique for analyzing complex data generated by higher-order polychromatic flow cytometry experiments 1 2 3 22 1 2 Janet C Siebert Lian Wang Daniel P Haley Ann Romer Bo Zheng Wes Munsil Kenton W Gregory Edwin B Walker3 Abstract Background The complex data sets generated by higher-order polychromatic flow cytometry experiments are a challenge to analyze. Here we describe Exhaustive Expansion a data analysis approach for deriving hundreds to thousands of cell phenotypes from raw data and for interrogating these phenotypes to identify populations of biological interest given the experimental context. Methods We apply this approach to two studies illustrating its broad applicability. The first examines the longitudinal changes in circulating human memory T cell populations within individual patients in response to a melanoma peptide gp100209-2M cancer vaccine using 5 monoclonal antibodies mAbs to delineate subpopulations of viable gp100-specific CD8 T cells. The second study measures the mobilization of stem cells in porcine bone marrow that may be associated with wound healing and uses 5 different staining panels consisting of 8 mAbs each. Results In the first study our analysis suggests that the cell surface markers CD45RA CD27 and CD28 commonly used in historical lower order 2-4 color flow cytometry analysis to distinguish memory from naive and effector T cells may not be obligate parameters in defining central memory T cells TCM . In the second study we identify novel phenotypes such as CD29 CD31 CD56 CXCR4 CD90 Sca1-CD44 which may characterize progenitor cells that are significantly increased in wounded animals as compared to controls. Conclusions Taken together these results demonstrate that Exhaustive Expansion supports thorough interrogation of complex .