tailieunhanh - Báo cáo hóa học: "Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation | Li and Kurlander Journal of Translational Medicine 2010 8 104 http content 8 1 104 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells Differing impact on CD8 T cell phenotype and responsiveness to restimulation Yixin Li Roger J Kurlander Abstract Background The ability to expand virus- or tumor-specific T cells without damaging their functional capabilities is critical for success adoptive transfer immunotherapy of patients with opportunistic infection or tumor. Careful comparisons can help identify expansion methods better suited for particular clinical settings and identify recurrent deficiencies requiring new innovation. Methods We compared the efficacy of magnetic beads coated with anti-CD3 and anti-CD28 anti-CD3 CD28 beads and soluble anti-CD3 plus mixed mononuclear cells designated a rapid expansion protocol or REP in expanding normal human T cells. Results Both anti-CD3 CD28 beads and soluble anti-CD3 promoted extensive expansion. Beads stimulated greater CD4 cell growth geometric mean of 56- versus 27-fold p at day 21 but both stimulated similar CD8 expansion 189- versus 186-fold . Phenotypically bead-treated CD4 and CD8 T cells and anti-CD3-treated CD4 cells typically assumed an effector effector memory phenotype by day 14. By comparison a subset of anti-CD3-treated CD8 cells derived from naive cells retained much greater expression of CD45RA CD27 and CCR7 than matched bead-treated cells despite comparable expansion. These cells were clearly distinguishable from CD45RA terminally differentiated effector cells by the presence of CD27 the absence of CD57 and their inability to produce cytokines after stimulation. When used to expand previously stimulated cells anti-CD3 plus autologous MNCs produced much less antigen-induced cell death of CD8 cells and significantly more CD8 expansion than beads. Conclusions Anti-CD3 CD28