tailieunhanh - Báo cáo hóa học: " Endotoxin and CD14 in the progression of biliary atresia"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Endotoxin and CD14 in the progression of biliary atresia | Chou et al. Journal of Translational Medicine 2010 8 138 http content 8 1 138 TRANSLATIONAL MEDICINE RESEARCH Open Access Endotoxin and CD14 in the progression of biliary atresia Ming-Huei Chou1 2 Jiin-Haur Chuang2 3 Hock-Liew Eng4 Ching-Mei Chen4 Chiou-Huey Wang5 Chao-Long Chen3 Tsun-Mei Lin1 5 6 Abstract Background Biliary atresia BA is a typical cholestatic neonatal disease characterized by obliteration of intra- and or extra-hepatic bile ducts. However the mechanisms contributing to the pathogenesis of BA remain uncertain. Because of decreased bile flow infectious complications and damaging endotoxemia occur frequently in patients with BA. The aim of this study was to investigate endotoxin levels in patients with BA and the relation of these levels with the expression of the endotoxin receptor CD14. Methods The plasma levels of endotoxin and soluble CD14 were measured with a pyrochrome Limulus amebocyte lysate assay and enzyme-linked immunosorbent assay in patients with early-stage BA when they received the Kasai procedure KP in patients who were jaundice-free post-KP and followed-up at the outpatient department in patients with late-stage BA when they received liver transplantation and in patients with choledochal cysts. The correlation of CD14 expression with endotoxin levels in rats following common bile duct ligation was investigated. Results The results demonstrated a significantly higher hepatic CD14 mRNA and soluble CD14 plasma levels in patients with early-stage BA relative to those with late-stage BA. However plasma endotoxin levels were significantly higher in both the early and late stages of BA relative to controls. In rat model the results demonstrated that both endotoxin and CD14 levels were significantly increased in liver tissues of rats following bile duct ligation. Conclusions The significant increase in plasma endotoxin and soluble CD14 levels during BA implies a possible involvement of endotoxin stimulated CD14

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