tailieunhanh - Báo cáo hóa học: " Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction | Sondergaard et al. Journal of Translational Medicine 2010 8 24 http content 8 1 24 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction ỉ I I I r c c I 1 7 Ch r l -J A I_I r r 2 I I r f r- s I l l- x rA -JI3 r I A X I p X I m zx V4 1 r . D r r A5AAi h l I_I mnr6 Claus S Sondergaard David A Hess Dustin J Maxwell Carla vveinneimer Ivana nosová Michael H Creer David Piwnica-Worms3 Attila Kovacs4 Lene Pedersen1 Jan A Nolta1 7 Abstract Human stem cells from adult sources have been shown to contribute to the regeneration of muscle liver heart and vasculature. The mechanisms by which this is accomplished are however still not well understood. We tested the engraftment and regenerative potential of human umbilical cord blood-derived ALDHhiLin- and ALDHloLin- cells following transplantation to NOD SCID or NOD SCID b2m null mice with experimentally induced acute myocardial infarction. We used combined nanoparticle labeling and whole organ fluorescent imaging to detect human cells in multiple organs 48 hours post transplantation. Engraftment and regenerative effects of cell treatment were assessed four weeks post transplantation. We found that ALDHhiLin- stem cells specifically located to the site of injury 48 hours post transplantation and engrafted the infarcted heart at higher frequencies than ALDHloLin- committed progenitor cells four weeks post transplantation. We found no donor derived cardiomyocytes and few endothelial cells of donor origin. Cell treatment was not associated with any detectable functional improvement at the four week endpoint. There was however a significant increase in vascular density in the central infarct zone of ALDHhiLin- cell-treated mice as compared to PBS and ALDHloLin- cell-treated mice. Conclusions Our data indicate that adult human stem cells do not become a significant .