tailieunhanh - Báo cáo hóa học: " Copy number and gene expression differences between African American and Caucasian American prostate cancer"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Copy number and gene expression differences between African American and Caucasian American prostate cancer | Rose et al. Journal of Translational Medicine 2010 8 70 http content 8 1 70 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Copy number and gene expression differences between African American and Caucasian American prostate cancer Amy E Rose1 Jaya M Satagopan2 Carole Oddoux3 Qin Zhou2 Ruliang Xu5 Adam B Olshen2 Jessie Z Yu1 Atreya Dash4 Jerome Jean-Gilles1 Victor Reuter6 William L Gerald6 Peng Lee 5 and Iman Osman 1 Abstract Background The goal of our study was to investigate the molecular underpinnings associated with the relatively aggressive clinical behavior of prostate cancer PCa in African American AA compared to Caucasian American CA patients using a genome-wide approach. Methods AA and CA patients treated with radical prostatectomy RP were frequency matched for age at RP Gleason grade and tumor stage. Array-CGH BAC SpectralChip2600 was used to identify genomic regions with significantly different DNA copy number between the groups. Gene expression profiling of the same set of tumors was also evaluated using Affymetrix HG-U133 Plus arrays. Concordance between copy number alteration and gene expression was examined. A second aCGH analysis was performed in a larger validation cohort using an oligo-based platform Agilent 244K . Results BAC-based array identified 27 chromosomal regions with significantly different copy number changes between the AA and CA tumors in the first cohort Fisher s exact test P . Copy number alterations in these 27 regions were also significantly associated with gene expression changes. aCGH performed in a larger independent cohort of AA and CA tumors validated 4 of the 27 15 most significantly altered regions from the initial analysis 3q26 5p15-p14 14q32 and 16p11 . Functional annotation of overlapping genes within the 4 validated regions of AA CA DNA copy number changes revealed significant enrichment of genes related to immune response. Conclusions Our data reveal molecular alterations