tailieunhanh - Báo cáo hóa học: "Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders | Buonaguro et al. Journal of Translational Medicine 2010 8 18 http content 8 1 18 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders 19 1 1 2 3 Luigi Buonaguro 1 Annacarmen Petrizzo Marialina Tornesello Maria Napolitano Debora Martorelli 9 II I k k I I f s2 V rd D 9 I I r -ý4 ỉ I I DA J D J 7r 5 rvy D k r I I s 6 I I I z í D Z S. m s z t I I 7 f 4- V c r I I 1 s 7 Giuseppe Castello Gerardo Beneduce Amalia De Renzo oreste Perrella Luca Romagnoli Vitor Sousa Valli De Re8 Riccardo Dolcetti3 Franco M Buonaguro1 Abstract Hepatitis C virus HCV is one of the major risk factors for chronic hepatitis which may progress to cirrhosis and hepatocellular carcinoma as well as for type II mixed cryoglobulinemia MC which may further evolve into an overt B-cell non-Hodgkin s lymphoma NHL . It has been previously shown that B-cell receptor BCR repertoire expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL is constrained to a limited number of variable heavy VH - and light VL -chain genes. Among these the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines. Introduction Hepatitis C virus HCV is a Hepacivirus of the Flaviviri-dae family mainly involved in hepatic disorders including chronic hepatitis which may progress to cirrhosis in about 10-20 of cases and further to hepatocellular carcinoma in 1-5 of cirrhotic patients 1 . Subsequently the virus has been implicated as one of the major risk .