tailieunhanh - Báo cáo hóa học: " Ezrin promotes invasion and metastasis of pancreatic cancer cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Ezrin promotes invasion and metastasis of pancreatic cancer cells | Meng et al. Journal of Translational Medicine 2010 8 61 http content 8 1 61 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Ezrin promotes invasion and metastasis of pancreatic cancer cells Yunxiao Meng Zhaohui Lu Shuangni Yu Qiang Zhang Yihui Ma Jie Chen Abstract Background Pancreatic cancer has a high mortality rate because it is usually diagnosed when metastasis have already occurred microscopic and gross disease . Ezrin plays important roles in cell motility invasion and tumor progression and it is especially crucial for metastasis. However its function in pancreatic cancer remains elusive. Methods and Results We found that ezrin overexpression promoted cell protrusion microvillus formation anchorage-independent growth motility and invasion in a pancreatic cancer cell line MiaPaCa-2 whereas ezrin silencing resulted in the opposite effects. Ezrin overexpression also increased the number of metastatic foci 6 8 vs. 1 8 in a spontaneous metastasis nude mouse model. Furthermore ezrin overexpression activated Erk1 2 in MiaPaCa-2 cells which might be partially related to the alteration of cell morphology and invasion. Immunohistochemical analysis showed that ezrin was overexpressed in pancreatic ductal adenocarcinoma PDAC and precancerous lesions . the tubular complexes in chronic pancreatitis CP and pancreatic intraepithelial neoplasm PanIN and respectively compared to normal pancreatic tissues 0 . Ezrin was also expressed in intercalated ducts adjacent to the adenocarcinoma which has been considered to be the origin of ducts and acini as well as the starting point of pancreatic ductal carcinoma development. Conclusions We propose that ezrin might play functional roles in modulating morphology growth motility and invasion of pancreatic cancer cells and that the Erk1 2 pathway may be involved in these roles. Moreover ezrin may participate in the early events of PDAC development and may promote its progression to