tailieunhanh - Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor | Kurzik-Dumke et al. Journal of Translational Medicine 2010 8 58 http content 8 1 58 RESEARCH JOURNAL OF TRANSLATIONAL MEDICINE Open Access In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor Ursula Kurzik-Dumke 21 Manuela Horner 1 2 Maria R Nicotra2 Michael Koslowski3 and Pier G Natali24 Abstract Background Htid encoded proteins are physiological partners of a wide spectrum of molecules relevant to neoplastic transformation. One of the molecular ligands of the cytosolic hTid-L and hTid-I forms is the ErbB-2 receptor variably over expressed in diverse solid tumors. Altered ErbB-2 signalling is associated with an unfavourable prognosis in about 30 of human breast malignancies. Methods We evaluated htid and HER-2 expression by quantitative real time PCR in tumors of different TNMG status and by immunohistochemistry in a cohort of breast tumors of the Luminal A B HER-2 and triple negative subtype. Results The RT-PCR analysis revealed that aberrant expression of all three htid forms correlates with malignant transformation. Furthermore elevated hTid-L expression can be associated with less aggressive tumors. The immunohistochemical testing revealed that tumors of the luminal A subtype are characterized by a high level of htid 81 . In contrast htid expression is significantly lower in tumors of the Luminal B 20 and HER-2 18 subtype over expressing the receptor and in the triple negative 40 more aggressive malignancies. A statistically significant inverse correlation between htid and ErbB-2 expression was found in human breast p 0 0001 and non-mammary tumors p 0 007 and in transgenic mice carrying the rat HER-2 neu oncogene. Conclusions Our findings provide in vivo evidence that htid is a tissue independent and evolutionarily conserved suppressor of ErbB-2. Background Htid-1 is the human counterpart 1 of the Drosophila melanogaster lethal 2 tumorous imaginal discs l 2 tid tumor suppressor .