tailieunhanh - báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells | Journal of Translational Medicine BioMed Central Research MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells Jiaqiang Ren Ping Jin Ena Wang Francesco M Marincola and David F Stroncek Address Department of Transfusion Medicine Clinical Center National Institute of Health 9000 Rockville Pike Bethesda Maryland 20892 USA Email Jiaqiang Ren - renj@ Ping Jin - PJin@ Ena Wang - EWang@ Francesco M Marincola - francesco_marincola@ David F Stroncek - DStroncek@ Corresponding author Open Access Published 23 March 2009 Received 25 January 2009 Journal of Translational Medicine 2009 7 20 doi 1479-5876-7-20 Accepted 23 March 2009 This article is available from http content 7 1 20 2009 Ren et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The unique features of human embryonic stem hES cells make them the best candidate resource for both cell replacement therapy and development research. However the molecular mechanisms responsible for the simultaneous maintenance of their self-renewal properties and undifferentiated state remain unclear. Non-coding microRNAs miRNA which regulate mRNA cleavage and inhibit encoded protein translation exhibit temporal or tissue-specific expression patterns and they play an important role in development timing. Results In this study we analyzed miRNA and gene expression profiles among samples from 3 hES cell lines H9 I6 and BG01v differentiated embryoid bodies EB derived from H9 cells at different time points and 5 adult cell types including Human Microvascular .