tailieunhanh - báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài :Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool | Journal of Translational Medicine BioMed Central Open Access Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR a versatile monitoring and discovery tool Elke Schultz-ThateC1 Daniel M Frey11 Daniela Margelli2 Nermin Raafat1 Chantal Feder-Mengus1 Giulio C Spagnoli1 and Paul Zajac 1 Address institute of Surgical Research and Hospital Management Dept. of Biomedicine University Hospital of Basel Basel Switzerland and 2Personnel Medical Service University Hospital of Basel Basel Switzerland Email Elke Schultz-Thater - eschultz@ Daniel M Frey - dfrey@ Daniela Margelli - dmargelli@ Nermin Raafat - nraafat@ Chantal Feder-Mengus - cfeder@ Giulio C Spagnoli - gspagnoli@ Paul Zajac - pzajac@ Corresponding author tEqual contributors Published 16 October 2008 Received 15 September 2008 Journal of Translational Medicine 2008 6 58 doi l479-5876-6-58 Accepted 16 October 2008 This article is available from http content 6 1 58 2008 Schultz-Thater et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Monitoring of cellular immune responses is indispensable in a number of clinical research areas including microbiology virology oncology and autoimmunity. Purification and culture of peripheral blood mononuclear cells and rapid access to specialized equipment are usually required. We developed a whole blood WB technique monitoring antigen specific cellular immune response in vaccinated or naturally sensitized individuals. Methods WB 300 pl was incubated at 37 C with specific antigens in the form of peptides or commercial vaccines for 5-16 hours. Following RNAlater addition to stabilize RNA the