tailieunhanh - báo cáo hóa học:" An intron 9 containing splice variant of PAX2"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học quốc tế đề tài : An intron 9 containing splice variant of PAX2 | Journal of Translational Medicine BioMed Central Research An intron 9 containing splice variant of PAX2 Antonia Busse Anika Rietz Stefan Schwartz Eckhard Thiel and Ulrich Keilholz Address Dept of Medicine III Charité Campus Benjamin Franklin Berlin Germany Email Antonia Busse - Anika Rietz - Stefan Schwartz - Eckhard Thiel - Ulrich Keilholz - Corresponding author Open Access Published 25 May 2009 Received 31 March 2009 Journal of Translational Medicine 2009 7 36 doi l479-5876-7-36 Accepted 25 May 2009 This article is available from http content 7 1 36 2009 Busse et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background PAX2 is a transcription factor with an important role in embryogenic development. However PAX2 expression was frequently identified in neoplasia responsible for the growth and survival of cancer cells. Due to alternative splicing of exon 6 exon 10 and exon 12 four isoforms of PAX2 are described so far. Methods The expression of an intron 9 containing PAX2 splice variant was analyzed in neoplastic B cell and solid tumor cell lines as well as in primary tumor samples by quantitative RT-PCR. PAX2 proteins were detected by Western Blot in a subset of cell lines. Results All 14 lymphoma cell lines expressed an undescribed PAX2 splice variant containing the entire intron 9 sequence and the exon 10 sequence. This splice variant could also be detected in 35 solid tumor cell lines in leukemia and lymphoma as well as in colon carcinoma and melanoma patient .