tailieunhanh - Báo cáo y học: "Systematic screening of polyphosphate (poly P) levels in yeast mutant cells reveals strong interdependence with primary metabolism"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Systematic screening of polyphosphate (poly P) levels in yeast mutant cells reveals strong interdependence with primary metabolism. | Open Access Research Systematic screening of polyphosphate poly P levels in yeast mutant cells reveals strong interdependence with primary metabolism Florian M Freimoser Hans Caspar Hurlimann Claude A Jakob Thomas P Werner and Nikolaus Amrhein Addresses institute of Plant Sciences ETH Zurich 8092 Zurich Switzerland. Institute of Microbiology ETH Zurich Zurich Switzerland. Correspondence Florian M Freimoser. Email ffreimoser@ Published 15 November 2006 Genome Biology 2006 7 RI09 doi gb-2006-7- 11-r109 The electronic version of this article is the complete one and can be found online at http 2006 7 11 R109 Received 2 August 2006 Revised 4 October 2006 Accepted 15 November 2006 2006 Freimoser et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Inorganic polyphosphate poly P occurs universally in all organisms from bacteria to man. It functions for example as a phosphate and energy store and is involved in the activation and regulation of proteins. Despite its ubiquitous occurrence and important functions it is unclear how poly P is synthesized or how poly P metabolism is regulated in higher eukaryotes. This work describes a systematic analysis of poly P levels in yeast knockout strains mutated in almost every non-essential gene. Results After three consecutive screens 255 genes almost 4 of the yeast genome were found to be involved in the maintenance of normal poly P content. Many of these genes encoded proteins functioning in the cytoplasm the vacuole or in transport and transcription. Besides reduced poly P content many strains also exhibited reduced total phosphate content showed altered ATP and glycogen levels and were disturbed in the secretion of acid phosphatase. .