tailieunhanh - Báo cáo y học: "The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. | Research Open Access The human urinary proteome contains more than 1500 proteins including a large proportion of membrane proteins Jun Adachi n Chanchal Kumar Yanling Zhang Jesper V Olsen and Matthias Mann Addresses Department of Proteomics and Signal Transduction Max-Planck Institute for Biochemistry Am Klopferspitz D-82152 Martinsried Germany. ỶCenter for Experimental Bioinformatics University of Southern Denmark Campusvej DK-5230 Odense M Denmark. Current address Graduate School of Global Environmental Studies Kyoto University Yoshida-Honmachi Sakyo-Ku Kyoto Japan. Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China. Correspondence Matthias Mann. Email mmann@ Published I September 2006 Genome Biology 2006 7 R80 doi I86 gb-2006-7-9-r80 The electronic version of this article is the complete one and can be found online at http 2006 7 9 R80 Received 30 May 2006 Revised 11 July 2006 Accepted I September 2006 2006 Adachi et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Urine is a desirable material for the diagnosis and classification of diseases because of the convenience of its collection in large amounts however all of the urinary proteome catalogs currently being generated have limitations in their depth and confidence of identification. Our laboratory has developed methods for the in-depth characterization of body fluids these involve a linear ion trap-Fourier transform LTQ-FT and a linear ion trap-orbitrap LTQ-Orbitrap mass spectrometer. Here we applied these methods to the analysis of the human urinary proteome. Results We employed one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and reverse phase .