tailieunhanh - Báo cáo y học: "The genome of Apis mellifera: dialog between linkage mapping and sequence assembly"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The genome of Apis mellifera: dialog between linkage mapping and sequence assembly. | Correspondence The genome of Apis mellifera dialog between linkage mapping and sequence assembly Michel Solignac Lan Zhang Florence Mougel Bingshan Li Dominique Vautrin Monique Monnerot Jean-Marie Cornuet Kim C Worley George M Weinstock and Richard A Gibbs Addresses Laboratoire Evolution Génomes et Speciation Centre National de la Recherche Scientifique 91198 Gif-sur-Yvette cedex France and University of Paris Sud 91405 Orsay France. Human Genome Sequencing Center Baylor College of Medicine Alkek 1519 One Baylor Plaza Houston TX 77030 USA. Centre de Biologie et de Gestion des Populations INRA CS 30016 Montferrier-sur-Lez 34988 Saint-Gély-du-Fesc France. Correspondence Michel Solignac. Email solignac@ Published 19 March 2007 Genome Biology 2007 8 403 doi gb-2007-8-3-403 The electronic version of this article is the complete one and can be found online at http 2007 8 3 403 2007 BioMed Central Ltd Abstract Two independent genome projects for the honey bee a microsatellite linkage map and a genome sequence assembly interactively produced an almost complete organization of the euchromatic genome. Assembly now includes 626 scaffolds that were ordered and oriented into chromosomes according to the framework provided by the third-generation linkage map AmelMap3 . Each construct was used to control the quality of the other. The co-linearity of markers in the sequence and the map is almost perfect and argues in favor of the high quality of both. Most eukaryotic genome sequencing projects are preceded by the construction of physical genetic and or cytological maps. For the honey bee genome project there was no physical map and because of the low resolution of the cytogenetic map the meiotic map was the only resource for organizing the sequence assembly on the chromosomes. The first generation map AmelMap1 comprised 541 markers on 24 linkage groups for 16 chromosomes 1 2 . Saturation was achieved by addition of 601 markers prepared